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KMID : 1102020200500010026
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Applied Microscopy
2020 Volume.50 No. 1 p.26 ~ p.26
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Stem cell behaviors on periodic arrays of nanopillars analyzed by high-resolution scanning electron microscope images
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Kang Ji-Hun
Kang Eun-Hye
Yun Young-Shik
Ji Seung-Muk
Yun In-Sik
Yeo Jong-Souk
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Abstract
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The biocompatible polyurethane acrylate (PUA) nanopillars were fabricated by soft lithography using three different sizes of nanobeads (350, 500, and 1000?nm), and the human adipose-derived stem cells (hASCs) were cultured on the nanopillars. The hASCs and their various behaviors, such as cytoplasmic projections, migration, and morphology, were observed by high resolution images using a scanning electron microscope (SEM). With the accurate analysis by SEM for the controlled sizes of nanopillars, the deflections are observed at pillars fabricated with 350- and 500-nm nanobeads. These high-resolution images could offer crucial information to elucidate the complicated correlations between nanopillars and the cells, such as morphology and cytoplasmic projections.
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KEYWORD
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Cell behavior, Human adipose-derived stem cell, Scanning electron microscope, Polyurethane acrylate, Nanopillar
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